Title | Knockout of the X-linked in the hypothalamic paraventricular nucleus impairs sympathetic output to brown fat and causes obesity. |
Publication Type | Journal Article |
Year of Publication | 2019 |
Authors | Sinden DS, Holman CD, Bare CJ, Sun X, Gade AR, Cohen DE, Pitt GS |
Journal | FASEB J |
Volume | 33 |
Issue | 10 |
Pagination | 11579-11594 |
Date Published | 2019 Oct |
ISSN | 1530-6860 |
Abstract | Fibroblast growth factor (FGF)13, a nonsecreted, X-linked, FGF homologous factor, is differentially expressed in adipocytes in response to diet, yet 's role in metabolism has not been explored. Heterozygous knockouts fed normal chow and housed at 22°C showed hyperactivity accompanying reduced core temperature and obesity when housed at 30°C. Those heterozygous knockouts showed defects in thermogenesis even at 30°C and an inability to protect core temperature. Surprisingly, we detected trivial FGF13 in adipose of wild-type mice fed normal chow and no obesity in adipose-specific heterozygous knockouts housed at 30°C, and we detected an intact brown fat response through exogenous β3 agonist stimulation, suggesting a defect in sympathetic drive to brown adipose tissue. In contrast, hypothalamic-specific ablation of recapitulated weight gain at 30°C. Norepinephrine turnover in brown fat was reduced at both housing temperatures. Thus, our data suggest that impaired CNS regulation of sympathetic activation of brown fat underlies obesity and thermogenesis in heterozygous knockouts fed normal chow.-Sinden, D. S., Holman, C. D., Bare, C. J., Sun, X., Gade, A. R., Cohen, D. E., Pitt, G. S. Knockout of the X-linked in the hypothalamic paraventricular nucleus impairs sympathetic output to brown fat and causes obesity. |
DOI | 10.1096/fj.201901178R |
Alternate Journal | FASEB J. |
PubMed ID | 31339804 |
PubMed Central ID | PMC6994920 |
Grant List | R01 HL071165 / HL / NHLBI NIH HHS / United States R01 DK056626 / DK / NIDDK NIH HHS / United States R01 HL122967 / HL / NHLBI NIH HHS / United States R01 DK103046 / DK / NIDDK NIH HHS / United States R37 DK048873 / DK / NIDDK NIH HHS / United States T32 GM007171 / GM / NIGMS NIH HHS / United States R01 HL112918 / HL / NHLBI NIH HHS / United States F30 HL131217 / HL / NHLBI NIH HHS / United States |