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Inducible Fgf13 ablation enhances caveolae-mediated cardioprotection during cardiac pressure overload.

TitleInducible Fgf13 ablation enhances caveolae-mediated cardioprotection during cardiac pressure overload.
Publication TypeJournal Article
Year of Publication2017
AuthorsWei EQ, Sinden DS, Mao L, Zhang H, Wang C, Pitt GS
JournalProc Natl Acad Sci U S A
Volume114
Issue20
PaginationE4010-E4019
Date Published2017 May 16
ISSN1091-6490
Abstract

The fibroblast growth factor (FGF) homologous factor FGF13, a noncanonical FGF, has been best characterized as a voltage-gated Na(+) channel auxiliary subunit. Other cellular functions have been suggested, but not explored. In inducible, cardiac-specific Fgf13 knockout mice, we found-even in the context of the expected reduction in Na(+) channel current-an unanticipated protection from the maladaptive hypertrophic response to pressure overload. To uncover the underlying mechanisms, we searched for components of the FGF13 interactome in cardiomyocytes and discovered the complete set of the cavin family of caveolar coat proteins. Detailed biochemical investigations showed that FGF13 acts as a negative regulator of caveolae abundance in cardiomyocytes by controlling the relative distribution of cavin 1 between the sarcolemma and cytosol. In cardiac-specific Fgf13 knockout mice, cavin 1 redistribution to the sarcolemma stabilized the caveolar structural protein caveolin 3. The consequent increase in caveolae density afforded protection against pressure overload-induced cardiac dysfunction by two mechanisms: (i) enhancing cardioprotective signaling pathways enriched in caveolae, and (ii) increasing the caveolar membrane reserve available to buffer membrane tension. Thus, our results uncover unexpected roles for a FGF homologous factor and establish FGF13 as a regulator of caveolae-mediated mechanoprotection and adaptive hypertrophic signaling.

DOI10.1073/pnas.1616393114
Alternate JournalProc. Natl. Acad. Sci. U.S.A.
PubMed ID28461495